Shoot organogenesis from cotyledons of sunflower

Indirect shoot organogenesis from leaf explants of Adhatoda vasica Nees

A novel protocol for indirect shoot organogenesis of Adhatoda vasica was developed using petiole explants derived from mature shrubby plants. Media with concentrations of cytokinins in combination with auxins were used to induce callus formation in two explants types: petiole and leaf segment. The frequency of callus formation from petiole and leaf segment explants on Murashige and Skoog (MS) b...

In vitro shoot organogenesis from Eucalyptus sp. leaf explants

Background In vitro organogenesis is one of the key techniques associated with genetic transformation, as it determines the successful regeneration of transgenic plants after co-cultivation with bacteria. Therefore, the development of efficient regeneration protocols is the most critical step in developing genetic transformation. Protocols have been developed for several species of eucalyptus. ...

Effects of Genotype and Cotyledon Section on Organogenesis in Sunflower

Effect of different metals on protease activity in sunflower cotyledons

Financial support: Grants from the Universidad de Buenos Aires (UBA) and from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (Argentina). Abbreviations: 2,4 DNPH: 2,4-dinitrophenylhydrazine AEBSF: 4-(2-aminoethyl) benzenesulfonyl fluoride cys: cysteine DAB: diaminobenzidine DNP: dinitrophenyl DTT: dithiothreitol HRP: horseradish peroxidase IAA: iodoacetamide PAGE: polyacry...

Direct Organogenesis and Plant Regeneration from Cotyledons of a Multipurpose Tree, Acacia mangium Willd

Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA. Full strength of MS medium supplemented with ...